Techniques for Biomolecule Characterisation
This short seminar discusses chromatographic techniques suitable for the analysis of large
molecular weight biomolecules. With an increased interest in the area of biotherapeutics and more
regulations focused on these pharmaceuticals, possible methods to characterise biomolecules are
required. Modes such as size exclusion (SEC), ion-exchange, hydrophobic interaction (HIC) and
affinity chromatography will be covered for intact biomolecules. Techniques that may be more
familiar to those analysts involved in small molecule analysis, such as reversed-phase LC-MS
methods for protein digests will also be discussed, alongside new tools focused primarily on large
molecules, for example SEC for monoclonal antibody monomer, fragment and aggregate separations.
Course attendees will leave with a better understanding of the variety of techniques available to
enable a more complete characterisation of biomolecules.
鈥� Biomolecule basics 鈥� Ion-exchange chromatography
鈥� Size exclusion chromatography 鈥� Hydrophobic interaction chromatography
鈥� Reversed-phase chromatography 鈥� Affinity chromatography
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
This short seminar discusses chromatographic techniques suitable for the analysis of large
molecular weight biomolecules. With an increased interest in the area of biotherapeutics and more
regulations focused on these pharmaceuticals, possible methods to characterise biomolecules are
required. Modes such as size exclusion (SEC), ion-exchange, hydrophobic interaction (HIC) and
affinity chromatography will be covered for intact biomolecules. Techniques that may be more
familiar to those analysts involved in small molecule analysis, such as reversed-phase LC-MS
methods for protein digests will also be discussed, alongside new tools focused primarily on large
molecules, for example SEC for monoclonal antibody monomer, fragment and aggregate separations.
Course attendees will leave with a better understanding of the variety of techniques available to
enable a more complete characterisation of biomolecules.
鈥� Biomolecule basics 鈥� Ion-exchange chromatography
鈥� Size exclusion chromatography 鈥� Hydrophobic interaction chromatography
鈥� Reversed-phase chromatography 鈥� Affinity chromatography
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
