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Fundamentals of HPLC

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Fundamentals of HPLC

CHROMacademy

Teaching method

Online

Subject areas

Analytical, Chromatography

Course level

Introductory

Country

UK

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Autosampler of gas chromatography

Course overview

This course will detail the fundamental aspects that are required to fully understand GC. We will focus on the theory of GC and the fundamental mechanisms that result in chromatographic separation. All aspects of the technology and system are covered - from gas supplies, through sample introduction and column chemistries, to an overview of common detector types. The main focus is on fundamental concepts, but important practical aspects and some basic troubleshooting will also be introduced. All of this will aid in day to day good practice and troubleshooting.

The material covered in this course includes:

Session 1 | Runtime - 75 min

  • Introduction to HPLC
  • Chromatographic Parameters
  • Reversed Phase HPLC - Introduction & Separation Mechanism
  • Reversed Phase HPLC - Solvents
  • Reversed Phase HPLC - Working with Ionizable Analytes

Session 2 | Runtime - 70 min

  • Split Injection
  • Splitless Injection
  • Other Sampling Techniques
  • Band Broadening

Session 3 | Runtime - 70 min

  • Stationary Phase Interactions
  • Stationary Phase Selection
  • Column Dimensions
  • Temperature Programming

Session 4 | Runtime - 70 min

  • Detectors
  • Flame Ionization Detector
  • Nitrogen Phosphorus Detector
  • Electron Capture Detector
  • Thermal Conductivity Detector

Each video session is accompanied by supporting modules from CHROMacademy and we will use the animations within CHROMacademy to clearly illustrate the science and processes that are being discussed. All four sessions should be completed in order to gain the full training. The final assessment must be completed in order to gain your certificate of completion.

Course Delivery & Duration

  • 4 video sessions each session is between 1-2 hours long
  • Tutor supported with access to our chromatography experts
  • Supporting training material
  • Course assessment with certificate

Our CHROMacademy tutors will deliver four video training sessions which you can access on demand. They will provide online support and tutoring where necessary.

Prerequisites

This course has been designed for novice HPLC users and those who have limited experience of HPLC operation. Everyone is welcome!

Additional information

Sign up for 1-year CHROMacademy Premier Membership ($469.00 / £349.00 / €419.00 per year) and you get access to this course and FIVE other video courses - as well as hundreds of multi-media online modules in Analytical Science.

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Teaching provider

Useful information

Venue: Maidstone Innovation Centre, Maidstone

Country: UK

Teaching method: Online

Duration: 8 hours

Cost: £349.00 Plus VAT

Language: English

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